Background. Although various rehabilitation methods have been used to enable patients to return to their normal activities after carpal tunnel release (CTR) such as low level laser therapy (LLLT) and median nerve mobilization, up till now, there is no evidence for the comparison of effects of these two techniques in patients with CTR. Purpose. To investigate and compare the effectiveness of LLLT and median nerve mobilization after CTR on the electrophysiological parameters of median nerve, pain, hand grip and pinch strength. Methods. Forty-five patients of both sexes with unilateral CTR participated in the study. They were randomly assigned into three equal groups. Group I received LLLT and group II received median nerve mobilization three sessions per week for five successive weeks. Group III was the control group. Electrophysiological parameters of the median nerve, visual analogue scale (VAS), hand grip and pinch strength were assessed pre, post-treatment and after three months (follow-up). Results. There was no significant difference between GI and GII in all variables at post-treatment and follow-up. However, there was significant difference in all variables at post-treatment in GI and GII as compared to GIII. At follow-up for all variables, there was no significant difference between GI and GIII (except for pain) with significant difference between GII and GIII. Conclusion. Both of neural mobilization and LLLT lead to better improvement in the electrophysiological parameters of the median nerve, pain and hand function after CTR (in favor of neural mobilization group). Also, median nerve mobilization is more effective on improving hand function than LLLT at follow-up.