Tag: Jan Talar

Membrane and cellular effects of red laser radiation on human erythrocytes sensitized with Zn-phthalocyanine

Ilya Zavodnik, Leu Zavodnik, Jolanta Kujawa, Jan Talar, Maria Bryszewska, Małgorzata Łukowicz, Iwona Pyszczek

Ilya Zavodnik, Leu Zavodnik, Jolanta Kujawa, Jan Talar, Maria Bryszewska, Małgorzata Łukowicz, Iwona Pyszczek – Membrane and cellular effects of red laser radiation on human erythrocytes sensitized with Zn-phthalocyanine. Fizjoterapia Polska 2002; 2(2); 145-153

Abstract
Background. There is increasing interest in photosensitizing dyes, used as effectors in photodynamic therapy and photosterilization of biological fluids. The plasma membrane of the cell appears to be the principal site of photodamage. The photohemolytic activity of the sensitizer can be used as a parameter of the dye’s photodynamic toxicity. The aim of the present study is to further characterize the mechanism(s) of cell damage caused by exposure to red laser (670 nm) emission in human erythrocytes sensitized by Zn-phthalocyanine, and to evaluate the role of different cell components in erythrocyte photostability. Material and methods. We measured the effect of various erythrocyte treatments on cell damage induced by laser irradiation: „light” and post-irradiated „dark” hemolysis, cellular glutathione oxidation, membrane lipid peroxidation, and membrane protein oxidation. Results. Laser irradiation of red blood cells sensitized by Zn-phthalocyanine caused the oxidation of membranous protein tryptophan and cysteine residues, fluidization of the membrane lipid bilayer, and lipid peroxidation. The rate of „light” hemolysis significantly increased with increasing pH from 6.8 to 8.5, and after cell membrane perturbations by alcohols or in the presence of dithiotreitol, which is thought to reduce thiol groups oxidized by photosensitization. Conclusions. Membrane lipid peroxidation, the thiol groups of membrane proteins, and intracellular glutathione oxidation do not participate in hemolysis induced by red light. Membrane band 3 proteins play a significant role in erythrocyte photostability.

Key words:
photosensitizing dyes, photohemolysis, photodynamic toxicity

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Ultrasonic wave-induced damage to human red blood cells

Iwona Pyszczek, Jan Talar, Jolanta Kujawa, Leu Zavodnik, Ewa Kilańczyk

Iwona Pyszczek, Jan Talar, Jolanta Kujawa, Leu Zavodnik, Ewa Kilańczyk – Ultrasonic wave-induced damage to human red blood cells. Fizjoterapia Polska 2002; 2(2); 134-139

Abstract
Background. The aim of this study was to evaluate the response of human red blood cells to physiotherapeutic ultrasound (1 MHz). Material and methods. The susceptibility of erythrocytes to ultrasonic wave-induced damage was indicated by cell hemolysis, changes in the electrochemical properties of cell membranes, and membrane-bound acetylcholinesterase activity. Results. Exposure of erythrocytes to ultrasound resulted in dose-dependent hemolysis, significant activation of membrane acetylcholineterase due to an increase in substrate affinity, slight membrane depolarization due to nonselective pore formation, and activation of membrane lipid peroxidation. Conclusions. These results show the significant biomodulation effect of the ultrasonic waves used in medical practice on human erythrocytes at the membrane and cellular level.

Key words:
physiotherapy, Hemolysis, acetylcholinestrerase

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The role of nitric oxide in the modulation of arterial smooth muscle contraction evoked by activation of adrenoreceptors during laser biostimulation

Magdalena Mackiewicz-Milewska, Jan Talar, Grzegorz Grześk, Leszek Szadujkis-Szadurski, Irena Bułatowicz, Zbigniew Śliwiński

Magdalena Mackiewicz-Milewska, Jan Talar, Grzegorz Grześk, Leszek Szadujkis-Szadurski, Irena Bułatowicz, Zbigniew Śliwiński – The role of nitric oxide in the modulation of arterial smooth muscle contraction evoked by activation of adrenoreceptors during laser biostimulation. Fizjoterapia Polska 2002; 2(2); 89-98

Abstract
Introduction. The mechanisms of action of laser biostimulation on tissue continues to be the object of scientific investigation. The positive impact of laser radiation of low and medium power on inhibiting vascular contraction has been proven. It remains unknown, however, exactly how this takes place. We investigated the role of nitric oxide in this process.Material and methods. An in vitro experiment was performed on isolated tail arteries of male Wistar rats. The arteries were first subjected to the effect of noradrenalin (NA) and phenylephrine (PHE), and then to the effect of laser radiation (10, 30 and 100 mW), Then an inhibitor of nitric oxide synthase was added to the incubation fluid, along with methylene blue, and the effects of laser radiation in these conditions were observed.Results. Laser radiation of 10, 30, and 110mW inhibits the vascular contraction reaction induced by NA and PHE. The addition of a nitric oxide inhibitor or a guanyl cyclase inhibitor (methylene blue) to the incubation fluid cancels the inhibitory effect of laser biostimulation on contraction. Conclusion. Our results imply that the inhibitory effect of laser biostimulation on vascular contraction is mediated by nitric oxide and its secondary transmitter, guanyl cyclase.

Key words:
laser biostimulation, vascular endothelium, Nitric Oxide, adrenoreceptors

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Rehabilitation for patients on renal replacement therapy: possibilities and prospects

Wirginia Tomczak-Watras, Adam Plewa, Jan Talar, Jacek Manitius

Wirginia Tomczak-Watras, Adam Plewa, Jan Talar, Jacek Manitius – Rehabilitation for patients on renal replacement therapy: possibilities and prospects. Fizjoterapia Polska 2002; 2(3); 245-249

Abstract
Achieving a high quality of life should be the basic treatment goal in chronic dialysis. The best means to achieve this aim is a rehabilitation program aimed at restoring the patient’s capacity to return to work, while in the case of older patients the primary goal is to attain functional independence in performing activities of daily living. The achievement of these goals makes renal replacement therapy cost-effective. In the future it will be necessary to constantly update, plan, and execute the rehabilitation program and to monitor the beneficial long-term outcomes of its application.

Key words:
Dialysis, Quality of Life, cost-effectiveness

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The gram-negative microorganisms isolated from patients treated at the department and clinic of rehabilitation at the Rydygier Medical University of Bydgoszcz

Eugenia Gospodarek, Beata Ulatowska, Ewa Kruszyńska, Jan Talar, Małgorzata Łukowicz

Eugenia Gospodarek, Beata Ulatowska, Ewa Kruszyńska, Jan Talar, Małgorzata Łukowicz – The gram-negative microorganisms isolated from patients treated at the department and clinic of rehabilitation at the Rydygier Medical University of Bydgoszcz. Fizjoterapia Polska 2002; 2(3); 209-215

Abstract
Background. The aim of the study was to evaluate the occurrence of Gram-negative bacteria isolated from patients hospitalised in the Department and Clinic of Rehabilitation at the Rydygier Medical University in Bydgoszcz.Materials and methods. The material of the analysis was 533 clinical samples collected from patients hospitalised in 2000-2001. The study included 366 Gram-negative bacterial strains isolated from clinical materials. Bacterial identification was based on their morphological characteristics. The species were identified with ID32E and ID32GN tests (bioMérieux). The isolates were screened for antimicrobial susceptibility by the disk-diffusion method.Results. It was isolated 366 strains, 101 (27.6%) in 2000 and 265 (72.4%) in 2001. The rods belonging to Enterobacteriaceae family were amounted to 65.0%, nonfermentative rods – 27.9%, Neisseria spp. – 4.1%, Haemophilus spp. – 1.6%, and Moraxella catarrhalis – 1.4%. The most frequently were identified Proteus spp. (25.4%), Escherichia coli (23.8%), Pseudomonas spp. (17.2%), Acinetobacter spp. (13.1%), Klebsiella spp. (7.4%), Serratia spp. (3.8%). All the Enterobacteriaceae strains were susceptible to imipenem. In 2000 91,1% of these rods were susceptible to cefepime and 81,7% in 2001. A percentage of the sensitive strains to norfloxacin reached in 2000 73.2%, to ciprofloxacin – 69,5%. In 2001 a percentage of the susceptible strains to quinolones decreased to about 50%. During that period among the nonfermentative rods to imipenem were susceptible 95.7% i 85.5% strains, to netilmicine – 65.2% i 62.7%, and to cefepime – 22.2% i 59.1%, respectively. We have found 38 (10.4%) strains which produced ESBLs. These enzymes the most often were identified in K. pneumoniae (31.6%) strains. The b-lactamase AmpC was affirmed in 10 (2.7%) strains. Conclusions. From among Gram-negative bacteria most frequent isolated the Enterobacteriaceae rods. The Proteus sp. and E. coli. strains were predominant bacteria. All strains of these bacteria were susceptible to imipenem.

Key words:
hospital infections, antibiotic resistance, Enterobacteriaceae, nonfermentic rods, ESBLs

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Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes

Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa

Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa – Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes. Fizjoterapia Polska 2002; 2(3); 203-208

Abstract
Background. The aim of this study was to investigate the effect of high – frequency continuous – wave ultrasound (1 MHz) on the haemolysis of human erythrocytes. Material and methods. Red blood cell suspensions in PBS were irradiated at 37°C for 10 min with 1 MHz continuous – wave ultrasound, using a range of intensities equivalent to those employed by ultrasonic physiotherapy equipment (0.3 – 1.5 W/cm2). Results. The temperature in the sample during sonication was measured. Erythrocyte exposure to continuous – wave ultrasound (ultrasonic intensity > 0,3 W/cm2) resulted in a significant cell haemolysis, accompanied by the temperature rise (41°C for 1,5 W/cm2). However, the heat alone did not induce red blood cells haemolysis. Conclusions. This suggests that mechanical component (acoustic cavitation) is responsible for the damage to the red blood cells after ultrasound irradiation.

Key words:
ultrasounds, haemolysis, temperature

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Electrophysiological tests in the evaluation of the central nervous system in patients with organic personality disturbances

Katarzyna Pawlak-Osińska, Henryk Kaźmierczak, Jan Talar, Beata Semrau

Katarzyna Pawlak-Osińska, Henryk Kaźmierczak, Jan Talar, Beata Semrau – Electrophysiological tests in the evaluation of the central nervous system in patients with organic personality disturbances. Fizjoterapia Polska 2003; 3(1); 56-61

Abstract
Background. Emotional disturbances and personality disorders most often result from pathologies of structure in the fronto-basal region and the temporal lobe. Psychological and organic causes are intertwined even within the scope of a single disease. The purpose of this research was to evaluate the suitability of electrophysiological indicators of organic brain damage in patients presenting with personality disorders of confirmed organic etiology.Material and methods. 40 patients were enrolled in the study, 22 with closed-head injuries and 18 with brain tumors diagnosed on the basis of imaging tests. In 4 cases, the neoplastic process involved the temporal lobe, in 10 cases the frontal lobe, in 2 cases the medial region, and in 2 patients the fronto-temporal region. The control group consisted of 40 healthy persons with no history of head injury, no chronic illness, and no otoneurological symptoms. All the subjects were tested with electronystagmography (ENG) and electroencephalography (EEG) with mapping. Results. The ENG tests revealed the greatest differences in the frequency of occurrence of pathological reactions in the form of eye-tracking nystagmus, which was much more often found in persons with head injuries. Spontaneous nystagmus with eyes open or closed and positional nystagmus appeared more often in persons with brain tumor. Pathologies in the EEG were found much more often in patients with post-traumatic syndrome, with a frequency that differed significantly from that of the brain tumor group. Conclusions. Pathologies detected in ENG and EEG tests were common in patients with organic personality disturbances. Asymmetry and qualitative disturbances in optokinetic nystagmus and the pendulum test, accompanied by abnormal results from superliminal caloric tests, were the most common pathological findings. Abnormal EEG co-occurred with personality disorders of both post-traumatic and neoplastic origin, but were distinctly more common in the closed-head injury group.

Key words:
personality disturbances, electronystagmography, electroencephalography

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Effect of high-frequency continuous – wave ultrasound on the osmotic stability of human erythrocytes

Iwona Pyszczek, Ilya B. Zavodnik, Jolanta Kujawa, Jan Talar

Iwona Pyszczek, Ilya B. Zavodnik, Jolanta Kujawa, Jan Talar – Effect of high-frequency continuous – wave ultrasound on the osmotic stability of human erythrocytes. Fizjoterapia Polska 2003; 3(1); 16-20

Abstract
Background. The aim of our research was to investigate the effect of high-frequency continuous-wave ultrasound (1 MHz) on the osmotic stability of human erythrocytes. Material and methods. Red blood cell suspensions in PBS were irradiated at 37°C for 10 min with 1 MHz continuous-wave ultrasound, using a range of intensities equivalent to those employed by ultrasonic physiotherapy equipment (0.5-1.5 W/cm2). After ultrasound exposure, the erythrocytes were incubated for 30 min in buffered NaCl solutions and osmotic hemolysis was measured. Results. Our results indicate that sonication induced an increase in the osmotic stability of human erythrocytes, especially at the ultrasonic intensity of 1.0 W/cm2. In this case a considerable increase in the critical hemolytic volume of erythrocytes was observed. Conclusions. These results point to a significant biomodulation effect of high-frequency continuous-wave ultrasound on human erythrocytes, especially at the membrane level.

Key words:
ultrasounds, human erythrocytes, osmotic stability, critical hemolytic volume

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Circadian changes of arterial blood pressure in obese subjects after-night-shift work

Jacek J. Klawe, Małgorzata Tafil-Klawe, Jan Talar, Wojciech Sikorski, Maciej Śmietanowski

Jacek J. Klawe, Małgorzata Tafil-Klawe, Jan Talar, Wojciech Sikorski, Maciej Śmietanowski – Circadian changes of arterial blood pressure in obese subjects after-night-shift work. Fizjoterapia Polska 2003; 3(2); 180-183

Abstract
Background. A number of papers have reported that blood pressure correlates positively with body weight in humans. The analysis of circadian variability of blood pressure in obese patients indicates an abnormal tone in regulatory mechanisms. The increased arterial blood pressure observed in healthy subjects after sleep deprivation suggests that in obese subjects there may be increased risk of arterial hypertension after night-shift work. Material and methods. The investigation was performed in 6 male obese subjects. 24-h blood pressure monitoring was performed in an isolation chamber where temperature and humidity were kept constant, after day- and night- shift work. Results. The values of mean arterial pressure during 24 h after night-shift work were significantly higher as compared with the values observed after daytime work (P < 0.001). Conclusions. 1. Obese subjects showed an intollerance of the night-work, manifesting by the increase in blond pressure of after night-work. 2. Night-work changes the circadian course of the blood pressure during 24 hours after night-work.

Key words:
shift work, arterial hypertension, obesity

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The impact of high-frequency continuous-wave ultrasound on the fluidity of human erythrocyte membranes

Iwona Pyszczek, Małgorzata Łukowicz, Marta Cychner, Jolanta Kujawa, Jan Talar

Iwona Pyszczek, Małgorzata Łukowicz, Marta Cychner, Jolanta Kujawa, Jan Talar – The impact of high-frequency continuous-wave ultrasound on the fluidity of human erythrocyte membranes. Fizjoterapia Polska 2003; 3(2); 175-179

Abstract
Background. The aim of this study was to investigate the effect of high-frequency continuous-wave ultrasound (1 MHz) on the fluidity of the lipid bilayer in human erythrocytes. Material and methods. Red blood cell suspensions in PBS (obtained from healthy donors) were irradiated at 37°C for 10 min with 1 MHz continuous-wave ultrasound, using a range of intensities equivalent to those employed by the ultrasonic devices used in physicotherapy (0.2-1.0 W/cm2). The fluidity of the lipid bilayer in human erythrocyte membranes was measured using a new method: spectrofluorimetry. Results. Our results indicate that sonication with ultrasonic intensity > 0.7 W/cm2 induced a slight decrease in the fluidity of the lipid bilayer in human erythrocytes. Conclusions. The reported results suggest that high-frequency continuous-wave ultrasound has a biomodulating impact on human erythrocytes, especially at the membrane level.

Key words:
ultrasounds, human erythrocytes, lipid bilayer fluidity

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