Aim. To determine the effect of ultraviolet on vitamin D activation and balance in postmenopausal woman. Materials and methods. This study was conducted on forty postmenopausal women suffering from vitamin D deficiency (diagnosed by physician), with their age ranged from 50 to 60 years. They were randomly assigned into two equal groups. Group (A) received ultraviolet for 12 weeks (n = 20), while group (B) served as a control group (n = 20). Vitamin D was evaluated by blood analysis while balance was evaluated by Biodex balance system before and after treatment for both groups. Results. Comparing both groups post-treatment showed that there was a statistically significant increase in vitamin D (p < 0.05) and statistically significant reduction in all balance indices (p < 0.05) in favor of group (A). Conclusions. Ultraviolet radiation is effective, easy to apply, simple and successful method in improving vitamin D concentration and balance in postmenopausal women.