Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes

Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa

Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa – Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes. Fizjoterapia Polska 2002; 2(3); 203-208

Abstract
Background. The aim of this study was to investigate the effect of high – frequency continuous – wave ultrasound (1 MHz) on the haemolysis of human erythrocytes. Material and methods. Red blood cell suspensions in PBS were irradiated at 37°C for 10 min with 1 MHz continuous – wave ultrasound, using a range of intensities equivalent to those employed by ultrasonic physiotherapy equipment (0.3 – 1.5 W/cm2). Results. The temperature in the sample during sonication was measured. Erythrocyte exposure to continuous – wave ultrasound (ultrasonic intensity > 0,3 W/cm2) resulted in a significant cell haemolysis, accompanied by the temperature rise (41°C for 1,5 W/cm2). However, the heat alone did not induce red blood cells haemolysis. Conclusions. This suggests that mechanical component (acoustic cavitation) is responsible for the damage to the red blood cells after ultrasound irradiation.

Key words:
ultrasounds, haemolysis, temperature

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Physicodiagnostic amalysis of the ozonotherapy effectiveness on not afflicted craniofacial skin

Danuta Lietz-Kijak, Paulina Strzelecka, Elżbieta Kubala, Kamil Kosko, Aleksandra Simińska, Barbara Stępniak, Artur Perz, Łukasz Kopacz, Marta Grzegocka, Marek Kiljański, Zbigniew Śliwiński

D. Lietz-Kijak, P. Strzelecka, E. Kubala, K. Kosko, A. Simińska, B. Stępniak, A. Perz, Ł. Kopacz, M. Grzegocka, M. Kiljański, Z. Śliwiński – Physicodiagnostic amalysis of the ozonotherapy effectiveness on not afflicted craniofacial skin. FP 2017; 17(1); 46-55

Abstract
Aim. Evaluation the impact factor of therapeutic agent in the form of ozone application to temperature and feeling on superficial skin on facial part of the skull.
Material and Methods. The study included 60 patients, healthy, not taking any medication, aged 20 – 30 years old, 30 women and 30 men. Each time, before diagnostic tests, on cleansed skin determined 3 points: A – Supramentale, B – Chelion and C – projection of head of condylar process
of mandible on surface of skin. Then proceeded to double application of ozone – 30-and 60-seconds, in accordance with current standards for extraoral areas. Between the applications and measurements of sensation and temperature respected 15-minute break. Sensation measurement was carried out using PulpoEndometr device, and temperature was tested with laser pointer thermometer (CA 380). The last element was noticing sensations of pain using VAS scale.
Results. The results were analyzed using Statistica software.
Conclusions. 30-seconds of application in point. A woman pointing to the initial symptoms of analgesic, but in the general population can be seen only trailers that action. 60-seconds
of application at this point, and both applications in point. B showed a slightly increased sensitivity to stimuli. Different applications of ozone impact were noted in point. C, which was a slight decrease
in sensitivity to stimuli. It is worth noting that both applications of ozone in men at point C show some symptoms of analgesic.

Key words:
ozontheraphy, analgesia, feeling, temperature, OzonyTron

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