M. Mackiewicz-Milewska, J. Talar, L. Szadujkis-Szadurski

M. Mackiewicz-Milewska, J. Talar, L. Szadujkis-Szadurski – The modulating effect of laser radiation on the alpha-adrenergic and serotoninergic signaling system in the tail artery of the rat. Fizjoterapia Polska 2001; 1(1); 17-22

Abstract
Introduction. Laser biostimulation has found wide application as an auxiliary method of treatment in many fields of medicine, yet the underlying mechanism of its effects remains unknown. We compared the effect of laser radiation on the contracture reaction in meningeal arteries evoked by means of the 5-HT3 ionotropic receptor with the impact on this reaction evoked by the activation of the metabotropic alpha-andrenergic receptor and the direct effect of the BAY K8644 agonist of Ca2+channels. We decided to perform these tests on the perfused tail artery of the rat.Material and methods. The tests were performed on Wistar rats. A cannula was introduced into the proximal segment of the artery and joined to a peristaltic pump. The distal end of the artery was loaded with a 500 mg weight and placed in a 20 ml vessel filled with oxygenated Kres fluid. After the control concentration-effect curve was ascertained the arteries were stabilized for 30 minutes, then subjected to 5 minutes of laser radiation of increasing strength. After each exposure the concentration-effect curve was noted for each of the tested agonists.Results. The results obtained point to the modulating effect of laser radiation on the increase in perfusion pressure caused by the stimulation of alpha-adrenergic receptors. Exposure to laser radiation in the interval from 10 mW to 110 mW has no impact on the maximum effect obtained after the application of 5-HT, nor after the application of BAY K8644.Conclusion. Contracture evoked by noradrenaline or phenylephrine is effectively restrained in a manner dependent on the duration and strength of the laser radiation. Our analysis indicates that the antagonistic effect of laser radiation in relation to NA and PHE is not associated with a change in the affinity of alpha-adrenergic receptors to agonists. This results from the lack of impact of laser radiation on the indicated KA values for NA and PHA.

Key words:
laser radiational, pha-adrenergic and serotoninergic system, tail artery of the rat

Invalid download ID.

Pobierz bezpłatnie artykuł w j. angielskim