Iwona Pyszczek, Ilya B. Zavodnik, Jolanta Kujawa, Jan Talar

Iwona Pyszczek, Ilya B. Zavodnik, Jolanta Kujawa, Jan Talar – Effect of high-frequency continuous – wave ultrasound on the osmotic stability of human erythrocytes. Fizjoterapia Polska 2003; 3(1); 16-20

Abstract
Background. The aim of our research was to investigate the effect of high-frequency continuous-wave ultrasound (1 MHz) on the osmotic stability of human erythrocytes. Material and methods. Red blood cell suspensions in PBS were irradiated at 37°C for 10 min with 1 MHz continuous-wave ultrasound, using a range of intensities equivalent to those employed by ultrasonic physiotherapy equipment (0.5-1.5 W/cm2). After ultrasound exposure, the erythrocytes were incubated for 30 min in buffered NaCl solutions and osmotic hemolysis was measured. Results. Our results indicate that sonication induced an increase in the osmotic stability of human erythrocytes, especially at the ultrasonic intensity of 1.0 W/cm2. In this case a considerable increase in the critical hemolytic volume of erythrocytes was observed. Conclusions. These results point to a significant biomodulation effect of high-frequency continuous-wave ultrasound on human erythrocytes, especially at the membrane level.

Key words:
ultrasounds, human erythrocytes, osmotic stability, critical hemolytic volume

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