Tag: hemoliza

Ultrasonic wave-induced damage to human red blood cells

Iwona Pyszczek, Jan Talar, Jolanta Kujawa, Leu Zavodnik, Ewa Kilańczyk

Iwona Pyszczek, Jan Talar, Jolanta Kujawa, Leu Zavodnik, Ewa Kilańczyk – Ultrasonic wave-induced damage to human red blood cells. Fizjoterapia Polska 2002; 2(2); 134-139

Abstract
Background. The aim of this study was to evaluate the response of human red blood cells to physiotherapeutic ultrasound (1 MHz). Material and methods. The susceptibility of erythrocytes to ultrasonic wave-induced damage was indicated by cell hemolysis, changes in the electrochemical properties of cell membranes, and membrane-bound acetylcholinesterase activity. Results. Exposure of erythrocytes to ultrasound resulted in dose-dependent hemolysis, significant activation of membrane acetylcholineterase due to an increase in substrate affinity, slight membrane depolarization due to nonselective pore formation, and activation of membrane lipid peroxidation. Conclusions. These results show the significant biomodulation effect of the ultrasonic waves used in medical practice on human erythrocytes at the membrane and cellular level.

Key words:
physiotherapy, Hemolysis, acetylcholinestrerase

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Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes

Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa

Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa – Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes. Fizjoterapia Polska 2002; 2(3); 203-208

Abstract
Background. The aim of this study was to investigate the effect of high – frequency continuous – wave ultrasound (1 MHz) on the haemolysis of human erythrocytes. Material and methods. Red blood cell suspensions in PBS were irradiated at 37°C for 10 min with 1 MHz continuous – wave ultrasound, using a range of intensities equivalent to those employed by ultrasonic physiotherapy equipment (0.3 – 1.5 W/cm2). Results. The temperature in the sample during sonication was measured. Erythrocyte exposure to continuous – wave ultrasound (ultrasonic intensity > 0,3 W/cm2) resulted in a significant cell haemolysis, accompanied by the temperature rise (41°C for 1,5 W/cm2). However, the heat alone did not induce red blood cells haemolysis. Conclusions. This suggests that mechanical component (acoustic cavitation) is responsible for the damage to the red blood cells after ultrasound irradiation.

Key words:
ultrasounds, haemolysis, temperature

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