Tag: low-level laser (LLL)

The impact of laser radiation (I=810 nm, P=100 mW) on acetylcholinesterase activity in human erythrocytes depending on the manner of energy dosage

Jolanta Kujawa, Magdalena Sadowska, Maria Bryszewska, Ireneusz Pieszyński, Mirosław Janiszewski

Jolanta Kujawa, Magdalena Sadowska, Maria Bryszewska, Ireneusz Pieszyński, Mirosław Janiszewski – The impact of laser radiation (I=810 nm, P=100 mW) on acetylcholinesterase activity in human erythrocytes depending on the manner of energy dosage. Fizjoterapia Polska 2004; 4(2); 136-142

Abstract

Background. The aim of this study was to investigate the impact of different doses and methods of applying low-power laser radiation (810 nm, 125 mW/cm2) on acetylcholinesterase (AChE) activity in human red blood cells. Material and methods. A suspension of human erythrocytes was irradiated with near-infrared laser light (810 nm, 125 mW/cm2) at different energy doses. AChE activity was measured by spectrophotometry. Km and Vmax were estimated with a Lineweaver-Burk graph, and compared with the results obtained for a non-irradiated control sample. Results. Near-infrared low-intensity laser radiation stimulated AChE activity. A fractionated dosage of energy with a larger dose applied first caused a statistically significant increase in AChE activity, more than the same dose applied continuously. Conclusions. Low-intensity near-infrared laser radiation caused AChE activity to change in a manner dependent on energy dose and the manner of energy application. The largest increase in the maximum velocity of the reaction was observed for continuous 9J. When fractionated doses (6J+ 3J and 9J+ 3J) were used, a larger increase in maximal reaction velocity was observed than for the continuous dose of 9 J or 12 J.

Key words:
low-level laser (LLL), fractionated doses, Vmax
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Laser biostimulation in the regeneration of the trigeminal nerve

Aneta Demidaś, Anna Koziatek, Ewa Boerner, Barbara Ratajczak, Maciej Kochański

Aneta Demidaś, Anna Koziatek, Ewa Boerner, Barbara Ratajczak, Maciej Kochański – Laser biostimulation in the regeneration of the trigeminal nerve. Fizjoterapia Polska 2004; 4(2); 129-135

Abstract

Background. Research on the effects of low-level laser (LLL) radiation on living organisms has been conducted for nearly 40 years now, and the effectiveness of LLL has been examined in many diseases. The aim of the present study was to examine the impact of laser biostimulation on the restoration of the sense of touch after injury to the mandibular and maxillary regions, using a double-blind experiment. Material and methods. Our research involved 61 patients with fractures of the upper or lower jaw. The subjects were divided into two groups: Group I (31 persons) received laser therapy, while Group II (30 persons) received a placebo treatment. Touch sensation was tested by a JVP Domes esthesiometer, and the surface areas of disturbed sensation were measured.Results. After the observation period, the surface area of diminished sensation was reduced by an average of 9.52 cm2 in the laser group, and 5.03 cm2 in the placebo group. The difference is statistically significant. A comparison of touch sensitivity in the two groups showed less sensitivity in the placebo group. The results of a questionnaire suggest that laser biostimulation has a positive impact on the regeneration of the sense of touch in the face.Conclusions. Low-level laser radiation accelerates regeneration of the trigeminal nerve.

Key words:
low-level laser (LLL), tactile sensation, mandibular injury, maxillary injury
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