Tag: Maria Bryszewska

The impact of low-power laser radiation (IR, λ = 810 nm) on ATP-ASE activity and the cell membrane structure of erythrocytes

Jolanta Kujawa, Iwona Pyszczek, Leu Zavodnik, Ilya Zavodnik, Vyacheslav Buko, Ewa Kilańczyk, Maria Bryszewska, Jan Talar

Jolanta Kujawa, Iwona Pyszczek, Leu Zavodnik, Ilya Zavodnik, Vyacheslav Buko, Ewa Kilańczyk, Maria Bryszewska, Jan Talar – The impact of low-power laser radiation (IR, λ = 810 nm) on ATP-ASE activity and the cell membrane structure of erythrocytes. Fizjoterapia Polska 2001; 1(3); 248-253

Abstract

Background. The biostimulatory and therapeutic effects of laser radiation at different wavelengths, power levels, and doses are well known, but the mechanisms underlying these effects are still not completely understood. The aim of our research was to investigate the effect of laser radiation on the function and structure of the cell membranes of human erythrocytes.Material and methods. Isolated erythrocyte cell membranes were irradiated with laser light (λ = 810 nm) of varying surface density of light energy and power. The activity of Na+, K+, and Mg2+, ATPases was used as the marker characterizing structural and functional changes in the cell membrane.Results. Laser radiation of wavelength λ = 810 nm was found to change the activity of the sodium-potassium pump in the cell membrane of human erythrocytes, while the nature of the changes turned out to be dependent on the radiation energy and power dose. The results obtained also indicate that laser irradiation induces an increase of lipid bilayer fluidity. No changes were observed in such parameters as cell stability, the level of lipid peroxidation products, reduced intracellular glutathione, or reduced oxyhemoglobin level.Conclusions. Our results indicate that laser radiation of wavelength λ = 810 nm, depending on power level and energy dosage, induces structural and functional changes in the cell membranes of human erythrocytes.

Key words:
biostimulation, ion pump, cell stability, Lipid Peroxidation, glutahione, Oxyhemoglobin
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Membrane and cellular effects of red laser radiation on human erythrocytes sensitized with Zn-phthalocyanine

Ilya Zavodnik, Leu Zavodnik, Jolanta Kujawa, Jan Talar, Maria Bryszewska, Małgorzata Łukowicz, Iwona Pyszczek

Ilya Zavodnik, Leu Zavodnik, Jolanta Kujawa, Jan Talar, Maria Bryszewska, Małgorzata Łukowicz, Iwona Pyszczek – Membrane and cellular effects of red laser radiation on human erythrocytes sensitized with Zn-phthalocyanine. Fizjoterapia Polska 2002; 2(2); 145-153

Abstract
Background. There is increasing interest in photosensitizing dyes, used as effectors in photodynamic therapy and photosterilization of biological fluids. The plasma membrane of the cell appears to be the principal site of photodamage. The photohemolytic activity of the sensitizer can be used as a parameter of the dye’s photodynamic toxicity. The aim of the present study is to further characterize the mechanism(s) of cell damage caused by exposure to red laser (670 nm) emission in human erythrocytes sensitized by Zn-phthalocyanine, and to evaluate the role of different cell components in erythrocyte photostability. Material and methods. We measured the effect of various erythrocyte treatments on cell damage induced by laser irradiation: „light” and post-irradiated „dark” hemolysis, cellular glutathione oxidation, membrane lipid peroxidation, and membrane protein oxidation. Results. Laser irradiation of red blood cells sensitized by Zn-phthalocyanine caused the oxidation of membranous protein tryptophan and cysteine residues, fluidization of the membrane lipid bilayer, and lipid peroxidation. The rate of „light” hemolysis significantly increased with increasing pH from 6.8 to 8.5, and after cell membrane perturbations by alcohols or in the presence of dithiotreitol, which is thought to reduce thiol groups oxidized by photosensitization. Conclusions. Membrane lipid peroxidation, the thiol groups of membrane proteins, and intracellular glutathione oxidation do not participate in hemolysis induced by red light. Membrane band 3 proteins play a significant role in erythrocyte photostability.

Key words:
photosensitizing dyes, photohemolysis, photodynamic toxicity

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The impact of laser radiation (I=810 nm, P=100 mW) on acetylcholinesterase activity in human erythrocytes depending on the manner of energy dosage

Jolanta Kujawa, Magdalena Sadowska, Maria Bryszewska, Ireneusz Pieszyński, Mirosław Janiszewski

Jolanta Kujawa, Magdalena Sadowska, Maria Bryszewska, Ireneusz Pieszyński, Mirosław Janiszewski – The impact of laser radiation (I=810 nm, P=100 mW) on acetylcholinesterase activity in human erythrocytes depending on the manner of energy dosage. Fizjoterapia Polska 2004; 4(2); 136-142

Abstract

Background. The aim of this study was to investigate the impact of different doses and methods of applying low-power laser radiation (810 nm, 125 mW/cm2) on acetylcholinesterase (AChE) activity in human red blood cells. Material and methods. A suspension of human erythrocytes was irradiated with near-infrared laser light (810 nm, 125 mW/cm2) at different energy doses. AChE activity was measured by spectrophotometry. Km and Vmax were estimated with a Lineweaver-Burk graph, and compared with the results obtained for a non-irradiated control sample. Results. Near-infrared low-intensity laser radiation stimulated AChE activity. A fractionated dosage of energy with a larger dose applied first caused a statistically significant increase in AChE activity, more than the same dose applied continuously. Conclusions. Low-intensity near-infrared laser radiation caused AChE activity to change in a manner dependent on energy dose and the manner of energy application. The largest increase in the maximum velocity of the reaction was observed for continuous 9J. When fractionated doses (6J+ 3J and 9J+ 3J) were used, a larger increase in maximal reaction velocity was observed than for the continuous dose of 9 J or 12 J.

Key words:
low-level laser (LLL), fractionated doses, Vmax
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