Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes
Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa
Iwona Pyszczek, Jan Talar, Marta Cychner, Jolanta Kujawa – Effect of high-frequency continous-wave ultrasound on the stability of human erythrocytes. Fizjoterapia Polska 2002; 2(3); 203-208
Abstract
Background. The aim of this study was to investigate the effect of high – frequency continuous – wave ultrasound (1 MHz) on the haemolysis of human erythrocytes. Material and methods. Red blood cell suspensions in PBS were irradiated at 37°C for 10 min with 1 MHz continuous – wave ultrasound, using a range of intensities equivalent to those employed by ultrasonic physiotherapy equipment (0.3 – 1.5 W/cm2). Results. The temperature in the sample during sonication was measured. Erythrocyte exposure to continuous – wave ultrasound (ultrasonic intensity > 0,3 W/cm2) resulted in a significant cell haemolysis, accompanied by the temperature rise (41°C for 1,5 W/cm2). However, the heat alone did not induce red blood cells haemolysis. Conclusions. This suggests that mechanical component (acoustic cavitation) is responsible for the damage to the red blood cells after ultrasound irradiation.
Key words:
ultrasounds, haemolysis, temperature
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