Oxidative stress in platelets subjected to the effects of the electromagnetic field occurring in motor vehicles

Andrzej Buczyński, Krzysztof Pacholski, Maria Dziedziczak-Buczyńska, Gabriela Henrykowska, Anna Jerominko

Andrzej Buczyński, Krzysztof Pacholski, Maria Dziedziczak-Buczyńska, Gabriela Henrykowska, Anna Jerominko – Oxidative stress in platelets subjected to the effects of the electromagnetic field occurring in motor vehicles. Fizjoterapia Polska 2004; 4(3); 254-258

Abstract

Background. New technologies introduced to improve the comfort of daily life may be associated with health hazards. Therefore, it is important to undertake studies to identify the potential sources of phenomena that may be hazardous for biological systems. The aim of the our study was to describe the processes by which reactive oxygen species are generated in blood platelets exposed to the magnetic field present in motor vehicles.Material and methods. The material consisted of human blood platelets in suspension. The samples were exposed to an electromagnetic field characterized by appropriate parameters in a specially designed experimental stand. After 30, 60 and 90 min, the chemiluminescence of the experimental and control samples was measured. The results were subjected to statistical analysis. Results. Significantly increased generation of free radicals was observed after 30 and 90 min of the experiment. The level of free radical generation after 60 min did not exceed the values obtained for the control sample.Conclusions. Electromagnetic radiation generated by a motor vehicle can significantly disturb the physiological functions of the organism of the vehicle driver and passengers, due to increased generation of free oxygen radicals.

Key words:
Free Radicals, chemilluminescence, Technology
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Evaluating the activity of catalase (CAT) and superoxide dismutase (SOD-1) in blood platelets exposed to the electromagnetic field emitted in automobiles

Andrzej Buczyński, Krzysztof Pacholski, Jan Talar, Maria Dziedziczak-Buczyńska, Anna Jerominko

Andrzej Buczyński, Krzysztof Pacholski, Jan Talar, Maria Dziedziczak-Buczyńska, Anna Jerominko – Evaluating the activity of catalase (CAT) and superoxide dismutase (SOD-1) in blood platelets exposed to the electromagnetic field emitted in automobiles. Fizjoterapia Polska 2004; 4(4); 385-390

Abstract

Background. Blood platelets play an important role in many physiological and pathological processes. Their dysfunction may lead to serious illness or death. Numerous exogenic factors may have an adverse impact on platelet metabolic processes by affecting enzymatic reactions. The aim of our study was to determine how catalase (CAT) and superoxide dismutase (SOD-1) are modified by the exogenic electromagnetic field (EMF) occurring in a motor vehicle.Material and methods. Suspensions of human blood platelets were used for this investigation. The suspensions were exposed to EMF with the appropriate characteristics in a specially prepared test stand. Catalase (CAT) and superoxide dismutase (SOD-1) activity were measured in the experimental suspensions and in a control group 30, 60 and 90 min after exposure. Results. A significant increase in catalase activity was observed 30 and 60 min after the exposure. The level of the investigated enzymes decreased 90 min after the exposure, as compared to the control group. A significant decrease in SOD-1 activity was observed at each time point in which the suspension was exposed to EMF, as compared to the control group.Conclusion. The registered changes in catalase oxidative activity point up the active role of this enzyme in platelet defense against the effects of the electromagnetic fields emitted by automobile electronics. The decreased platelet antioxidative efficiency noted during the experiment may be connected with SOD-1 activity reduction.

Key words:
enzymatic reactions, platelet metabolism, anti-oxidative action
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